Relative level of total CYP2D6 between the purified CYP2D6.2 and CYP2D6.1 calculated from representative matched peptides by DeCyder MS
After characterization with BCA protein assay, CO difference spectrum, and immunoblot analysis, equal amount (holoprotein + apoprotein) of CYP2D6.2 and CYP2D6.1 was used for the analysis.
Peptide IDa | Retention Time | Peptide Sequence | CYP2D6.2/CYP2D6.1 Ratio | Xcorr |
|---|---|---|---|---|
| min | ||||
| 130 | 34.2 | R.MTWDPAQPPR.D | 1.22 | 3.14 |
| 556 | 43.2 | R.FGDIVPLGVTHMTSR.D | 1.11 | 3.38 |
| 473 | 43.3 | K.DEAVWEKPFR.F | 1.23 | 3.31 |
| 253 | 50.2 | R.LLDLAQEGLK.E | 1.34 | 2.61 |
| 214 | 52.1 | R.VQQEIDDVIGQVR.R | 1.39 | 5.25 |
| 205 | 60.0 | R.FGDIVPLGVTHMTSR.D | 1.22 | 2.69 |
| 476 | 63.1 | K.AVSNVIASLTCGR.R | 1.45 | 2.79 |
| 112 | 87.2 | K.GTTLITNLSSVLK.D | 1.28 | 4.20 |
| 141 | 93.3 | R.PPVPITQILGFGPR.S | 1.19 | 2.61 |
| Mean ± S.D. |
|
| 1.27 ± 0.11 |
|
↵ a A unique ID for each matched peptide assigned by the DeCyder MS “Pepmatch” module.