The primers used for amplification and sequencing of exons and for directed mutagenesis
Unless otherwise specified, either PCR primer was used for sequencing. In the primer sequence for directed mutagenesis, a nucleotide to be changed is indicated in lowercase.
Exon/Variant | Sequence (5′ → 3′) | ||
|---|---|---|---|
| For amplification and sequencing of exons: | |||
| Exon 2 | F | CCCTGTGATAAGTAGAGGCTGA | |
| R | CACTTATGATTCTCAATTTCTTC | ||
| Exon 3 | F | GAAAAGCCACCCTGAAA | |
| R | TGCCTGGCCTAAGTATTTG | ||
| S | TTCAGCCATCTCATATTTTGT | ||
| Exon 5 | F | GTGATTGGCCTGAAGTTGC | |
| R | GCCCCCAGATAGGAATCAG | ||
| Exon 6 | F | CTGACTGGCTTAGTGGGTTAG | |
| R | CCATCGCAGGAGAATAATG | ||
| S | TCTTCACCCATGCACACTCTA | ||
| Exon 7 | F | GAGGATTGAAGTCATGGTG | |
| R | GGCAGAGTGTGAGCTATCA | ||
| Exon 9 | F | CAATGTGCATGACAGAAAAG | |
| R | TGCCTAGACAGTAGATAGGAGTG | ||
| For directed mutagenesis: | |||
| c.449TG>A | F | CCGAATTCAAGAGGAACCTaTGTCTGGTGGAAG | |
| R | CTTCCACCAGACAtAGGTTCCTCTTGAATTCGG | ||
| c.707A>T | F | GGAAGTCATAATGTGTTTTTAAAAATTtTGCTCCCAAAGAAG | |
| R | CTTCTTTGGAGAGCAaATTTTTAAATAACACATATGACTTCC | ||
| c.883A>G | F | CTTGGTTGCTACTATATGGATATGTTTgGTGCAGAACAGAG | |
| R | CTCTGTTCCTGCACcAACATATCCCATATAGTACAACCAAG | ||
| c.1292A>G | F | GCTTTTTCAGCAGGAAgAGAATTTGTGCTGGAGAAGGC | |
|
| R | GCCTTCTCCAGCACAAATCTTcTTCCTGCTGAAAAAGC | |
F, forward primer; R, reverse primer; S, sequence primer.