TABLE 2

UGTs involved in glucuronidation of VPA in vitro

Glucuronidation activity was measured in eight UGT Supersomes at different concentrations of VPA. Values are represented as means of triplicate measurements ± S.D.

UGT Enzyme	V_max	K_m	Cl_int
	nmol/min/mg	mM	μl/min/mg
UGT1A1	N.A.	N.A.
UGT1A3	0.6 ± 0.3	6.4 ± 0.76	0.09
UGT1A4	0.4 ± 0.1	3.1 ± 0.32	0.13
UGT1A6	0.70 ± 0.44^a	3.2 ± 0.53^a	0.22^a
UGT1A8	0.87 ± 0.5	5.9 ± 0.76	0.14
UGT1A9	0.96 ± 0.5	6.3 ± 0.75	0.15
	0.98 ± 0.06^a	5.2 ± 0.84^a	0.19^a
UGT1A10	0.88 ± 0.3	5.5 ± 0.39	0.16
UGT2B7	1.2 ± 0.15	1.4 ± 0.09	0.86
	0.66 ± 0.44^a	2.1 ± 0.35^a	0.31^a

N.A., no activity.
↵ a Denotes literature-reported values (Ethell et al., 2003).