Michealis-Menten kinetics in five HLMs from elderly and younger donor bank
Values are represented as means of triplicate measurements ± S.D.
HLM No. | Vmax | Km | Clint = Vmax/Km |
|---|---|---|---|
| nmol/min/mg | mM | μl/min/mg | |
| EHLM 1 | 44 ± 2.8 | 0.8 ± 0.2 | 55 |
| EHLM 2 | 64 ± 3.0 | 1.1 ± 0.4 | 58.2 |
| EHLM 3 | 32 ± 2.7 | 0.6 ± 0.3 | 53.3 |
| EHLM 4 | 82 ± 2.8 | 2.5 ± 0.6 | 32.8 |
| EHLM 5 | 59 ± 0.9 | 0.9 ± 1.0 | 65.6 |
| Avg. | 56.2 ± 19.2 | 1.2 ± 0.8 | 52.98 ± 12.2 |
| YHLM 1 | 37 ± 2.4 | 0.7 ± 0.3 | 52.9 |
| YHLM 2 | 45 ± 2.8 | 0.8 ± 0.2 | 56.3 |
| YHLM 3 | 82 ± 3.5 | 2.4 ± 0.5 | 34.2 |
| YHLM 4 | 85 ± 1.4 | 2.5 ± 0.2 | 34 |
| YHLM 5 | 34 ± 2.0 | 0.7 ± 0.6 | 48.6 |
| Avg. | 56.62 ± 24.9 | 1.4 ± 0.9 | 45.2 ± 10.5 |
| Pooled HLMs | 32.6 ± 0.6 | 2.4 ± 0.4 | 13.9 |