Forward mutagenic primers used for site-directed mutagenesis
Name | Mutation | Primer | Sequence |
|---|---|---|---|
| Mut 1 | R42Q | Mut 1 | 5′-G AGC CAC TGG TTC ACC ATG CAG TCG GTG GTG GAG AAA CTC ATT C-3′ |
| Mut 2 | K91M, A92D | Mut 2 | 5′-GAT CTG GAC CGG GAG TTC ATG GAT TTT GCC CAT GCT CAA TG-3′ |
| Mut 3 | Y106F, G111S | Mut 3-1 | 5′-CA CAA GTA CGA AGT ATA TTT TCT CTA TTA ATG AGT TCA TAC AAT G-3′ |
| D115G | Mut 3-2 | 5′-G GGT TCA TAC AAT GGT ATT TTT GAC TTA TTT TTT TCA AAT TGC AGG-3′ | |
| Mut 4 | N152A | Mut 4 | 5′-CA GTG TTT CTC GAT CCT TTT GAT GCC TGT GGC TTA ATT GTT GCC-3′ |
| Mut 5 | L173A | Mut 5 | 5′-GCT TTC GCC AGG GGA ATA GCT TGC CAC TAT CTT GAA GAA GGT G-3′ |
| Mut 6 | L219F, H221Q, R222Y | Mut 6 | 5′-G CAC TTG GAG GAA CAT TTA TTT TGC CAG TAT TTT TTC AAA AAT GC-3′ |
| Mut 7 | E241A | Mut 7 | 5′-CTC CAA ACA CCT GTT ACG GCA TAT GAT CTC TAC AGC CAC AC-3′ |
Mutated sites are indicated by underlined bold letters.