Km | Vmax | Vmax/Km | |
---|---|---|---|
μM | nmol/min/unit | μl/min/unit | |
Supersomes | |||
UGT1A9 | 13.9 ± 0.8 | 0.5 ± 0.05 | 33 ± 2 |
UGT1A7 | 268.7 ± 27.8 | 0.2 ± 0.08 | 0.59 ± 0.18 |
UGT1A8 | >10000 | ||
UGT1A10 | 1966.7 ± 723.4 | 0.03 ± 0.001 | 0.015 ± 0.004 |
HEK293 cells | |||
Wild-type | 16.9 ± 1.2 | 1.0 ± 0.1 | 58 ± 4 |
UGT1A9 | |||
Mut 1 | 30.0 ± 4.3* | 1.1 ± 0.1 | 38 ± 5 |
Mut 2 | 17.8 ± 6.4 | 0.7 ± 0.06 | 45 ± 16 |
Mut 3 | 10.2 ± 5.7 | 1.6 ± 0.1** | 192 ± 100** |
Mut 4 | 49.2 ± 2.7** | 2.0 ± 0.1** | 41 ± 4 |
Mut 5 | 15.7 ± 0.8 | 1.5 ± 0.3** | 96 ± 23 |
Mut 6 | 15.2 ± 5.3 | 0.6 ± 0.08* | 41 ± 9 |
Mut 7 | 18.8 ± 5.4 | 0.6 ± 0.04* | 32 ± 12 |
↵* P < 0.05 and ** P < 0.01 compared with wild-type UGT1A9.