TABLE 1

Incubation validation summary showing optimal protein concentration, fu, and percentage enzyme activity after 60-min incubation stress as change of initial reaction rate

Values shown as mean with S.D. (n = 3).


Substrate

Optimal Protein Concentration

fu

Enzyme Activity at 60 Min
mg ml-1 %
Benzyloxyresorufin 0.50 0.21 ± 0.02 34.3 ± 2.9
Diclofenac 0.50 0.85 ± 0.05 79.7 ± 2.1
Dextromethorphan 0.50 0.71 ± 0.08 75.5 ± 9.6
Ethylmelagatran 0.30 1.0 ± 0.11 129 ± 12
Ethoxyresorufin 0.125 0.61 ± 0.04 101 ± 7.8
Flutamide 0.50 0.68 ± 0.02 85.1 ± 3.8
N-Hydroxymelagatran
0.30
0.90 ± 0.17
126 ± 16