TABLE 3

Summary of the Monte Carlo simulation study investigating the robustness of the multiple depletion curves method

Accuracy and precision (estimated value/true value ± coefficient of variation) of the estimated kinetic variables, Vmax and Km, at different relative initial concentration levels, C0/Km (high-medium-low), and relative turnover (% min–1).



Relative Turnover (% min-1)
5
0.5
0.05
C0/Km (high-medium-low)
Km
Vmax
Km
Vmax
Km
Vmax
20-2-0.2 1.0 ± 0.088 1.0 ± 0.077 1.0 ± 1.3 1.0 ± 1.1 0.99 ± 16 0.97 ± 13
2-0.2-0.02 1.0 ± 1.2 1.0 ± 1.0 0.98 ± 17 0.97 ± 15 14 ± 670 9.5 ± 680
0.2-0.02-0.002
1.2 ± 43
1.1 ± 41
6.9 ± 770
5.4 ± 730
*
*
  • * Estimation not possible.