TABLE 1

NADPH-cytochrome P450 reductase and cytochrome-b5 content in a set of 46 HLMs

Values are mean ± S.D. The young group included liver donors ≤45 years old, and the elderly group included donors ≥46 years old [n = 21 (young males), n = 11 (elderly males), n = 7 (young females), and n = 7 (elderly females)].

Assay Method
Young Males
Elderly Males
Young Females
Elderly Females
nmol/mg
NADPH-cytochrome P450 reductase content
    Spectrophotometric 0.095 ± 0.033* 0.069 ± 0.037* 0.082 ± 0.031 0.095 ± 0.039
    Immunoblot 0.033 ± 0.014* 0.019 ± 0.011* 0.025 ± 0.017 0.026 ± 0.016
Cytochrome-b5 content
    Spectrophotometric 0.35 ± 0.16* 0.20 ± 0.16* 0.40 ± 0.15 0.31 ± 0.22
    Immunoblot
1.44 ± 0.54*
0.76 ± 0.39*
0.79 ± 0.37
1.28 ± 0.35

  • ↵* P < 0.05 for young males versus elderly males by Student-Newman-Keuls test.