TABLE 1

Real-time PCR assays of VDR mRNA expression in Caco2, HEK293, HepG2, primary human hepatocytes and mouse livers

Primary human hepatocytes were isolated from seven human donors (indicated by HH#). Two micrograms of mRNA of Caco2, HEK293, HepG2, human hepatocytes, or mouse livers were used for the reverse-transcription reaction. UBC mRNA expression was monitored as the internal control. Ct values of VDR and UBC were measured by quantitative real-time PCR. Assays were done in triplicate. ΔCt was calculated by subtracting Ct of UBC from Ct of VDR. The S.D. of ΔCt = square root [(SD of Ct of UBC)² + (SD of VDR)²].