Testosterone and epitestosterone glucuronidation activity screen of all the recombinant human UGTs of subfamilies 1A, 2A, and 2B
Values are averages of three replicates ± S.D. Rates were not corrected for expression level.
UGT | Glucuronide formation | ||
|---|---|---|---|
| Testosterone | Epitestosterone | ||
| pmol/mg of protein/min | |||
| 1A1 | n.d. | n.d. | |
| 1A3 | 4.6 ± 0.3 | n.d. | |
| 1A4 | 5.5 ± 0.3 | 4.9 ± 0.8 | |
| 1A5 | n.d. | n.d. | |
| 1A6 | n.d. | n.d. | |
| 1A7 | n.d. | n.d. | |
| 1A8 | 2.7 ± 0.1 | n.d. | |
| 1A9 | 10.4 ± 0.5 | n.d. | |
| 1A10 | 7.9 ± 0.8 | n.d. | |
| 2A1 | 253 ± 5.4 | 172 ± 0.9 | |
| 2A2 | 13.5 ± 0.5 | 69.7 ± 5.4 | |
| 2A3 | n.d. | n.d. | |
| 2B4 | n.d. | 8.1 ± 0.6 | |
| 2B7 | 4.6 ± 0.1 | 312.5 ± 20.0 | |
| 2B10 | n.d. | n.d. | |
| 2B11 | n.d. | n.d. | |
| 2B15 | 7.9 ± 0.1 | n.d. | |
| 2B15ca | 63 ± 2.9 | n.d. | |
| 2B17 | 508.9 ± 19.6 | n.d. | |
| 2B28 | n.d. | n.d. | |
n.d., no detectable activity.
↵ a UGT2B15 Supersomes.