TABLE 1

Canine P450 and reductase expression yields using shake flask and fermenter cultures

Total P450 Yield
Specific P450 Contenta
P450 Reductase Contenta,b
Enzyme
Flask
Fermenter
Flask
Fermenter
Flask
Fermenter
nmol/l culture pmol/mg pmol/mg
CYP1A1 108 74 545 1416 95 158
CYP2B11 52 12 309 307 126 309
CYP2C21 44 17 198 303 83 124
CYP2C41 59 6 316 133 141 105
CYP2D15 55 7 242 381 82 171
CYP3A12 14 7 74 198 73 53
CYP3A26
4.8
0.16
48
29
30
45

  • ↵ a Specific enzyme content and activity of E. coli membranes from fermenter cultures were determined after additional centrifugation and filtering steps as described under Materials and Methods.

  • ↵ b P450 reductase content was estimated from cytochrome c reductase activity (Parikh et al., 1997).