Comparison of cytokine effects on transporter mRNA expression in human hepatocytes
Transporter | Effects of Cytokine Treatmenta | ||||
|---|---|---|---|---|---|
| TNF-α | IL-6 | IL-1βb | |||
| (n = 6) | (n = 6) | (n = 6) | |||
| MDR1 | ↔c | ↓ d(1.6) | ↓ (1.7) | ||
| BSEP | ↓ (8.0) | ↔ | ↓ (7.1) | ||
| MRP2 | ↔ | ↓ (1.8) | ↓ (2.3) | ||
| MRP3 | ↔ | ↔ | ↓ (1.9) | ||
| MRP4 | ↔ | ↓ (1.7) | ↓ (1.8) | ||
| BCRP | ↔ | ↓ (3.2) | ↓ (3.7) | ||
| NTCP | ↓ (2.9) | ↓ (18.8) | ↓ (11.1) | ||
| OATP2B1 | ↓ (1.7) | ↓ (3.8) | ↓ (6.0) | ||
| OATP1B1 | ↓ (3.1) | ↓ (6.5) | ↓ (8.8) | ||
| OATP1B3 | ↓ (3.6) | ↓ (3.3) | ↓ (8.1) | ||
| OCT1 | ↓ (2.8) | ↓ (2.9) | ↓ (4.9) | ||
| OAT2 | ↓ (4.7) | ↓ (2.5) | N.D.e | ||
↵ a Human hepatocytes were exposed to cytokines for 24 h. mRNA transporter expression was then determined by RT-qPCR.
↵ c ↔, no significant changes in mRNA levels when compared with untreated control hepatocytes.
↵ d ↓, significant repression of mRNA levels when compared with untreated control cells; numbers in parentheses indicate the repression factor, i.e., the ratio of mRNA levels in untreated cells versus those found in cytokine-exposed cells.
↵ e N.D., not done.