Kinetic parameters for dextromethorphan O-demethylation catalyzed by human liver microsomes with different CYP2D6 genotypes
Dextromethorphan (10-400 μM) was incubated with human liver microsomes from subjects genotyped as shown in the table. Kinetic parameters were calculated by nonlinear regression analysis.
CYP2D6 Genotype | Km a | Vmax a | Vmax/Km |
|---|---|---|---|
| μM | pmol/min/mg protein | μl/min/mg protein | |
| *1/*1 (n = 3) | 10.2 ± 1.5 | 157 ± 77 | 15.4 |
| *1/*10 (n = 3) | 15.3 ± 9.1 | 120 ± 14 | 7.8 |
| *10/*10 (n = 3) | 23.9 ± 8.2 | 148 ± 103 | 6.2 |
| *10/*49 (n = 1) | 39.7 | 141 | 3.6 |
↵ a Data are represented as mean ± S.D. of liver microsomes from different individuals.