TABLE 1

Molecular masses of wild-type and monomeric mutant (V270E) of SULT1A1

SULT1A1
Ve a
Apparent Mrb
Theoretical Mrc
ml Da
Wild-type 52.53 63093 68,331
V270E
62.69
37350
34,195

  • ↵ a Ve indicates the retention volume of Sephacryl S-200 HR column.

  • ↵ b The apparent molecular masses were determined according to the calibration curve by Sephacryl S-200 HR column chromatography.

  • ↵ c The theoretical molecular masses were calculated by the ProtParam on the ExPASy server (http://tw.expasy.org/tools/protparam.html).