Immunoassay (immunohistochemistry, RIA, ELISA, and immunoblot) | • Distribution, identification, and quantification in tissue and cellular preparations |
• Enzyme induction | |
• Enzyme mechanism | |
• Toxicity (enzyme level induction) | |
• Risk assessment (cancer susceptibility) | |
• Membrane topography | |
Immunoaffinity | • Purification, precipitation, and production |
Immunoinhibition | • Reaction phenotyping: quantitative contribution of a single or multiple P450s to the metabolism of a drug or toxin (product formation or substrate disappearance) |
• DDI prediction (fm, CYP) | |
• Pharmacogenetics (phenotypic polymorphism) | |
• Toxicity (toxicant formation, tissue, and cell toxicity) | |
| • Bioactivation (mutagenesis, carcinogenesis, DNA, and protein adduct formation) |