Uptake of different OCT2 substrates in HEK cells stably expressing wild-type OCT2 or the OCT2 p0.270Ala>Ser mutant protein
Each value represents the mean ± S.E.M. of n determinations from 2 to 3 independent experiments.
n | Km | Vmax | |||||
|---|---|---|---|---|---|---|---|
| OCT2 Wild-Type | OCT2 p.270Ala>Ser | OCT2 Wild-Type | OCT2 p.270Ala>Ser | ||||
| μM | pmol/mg/min | ||||||
| MPP+ | 6 | 19.5 ± 2.8 | 20.9 ± 4.3 | 579 ± 17 | 414 ± 18*** | ||
| Dopamine | 8 | 932 ± 77 | 1285 ± 132* | 7559 ± 173 | 6820 ± 203* | ||
| Norepinephrine | 12 | 5452 ± 498 | 5282 ± 907 | 2135 ± 90 | 1671 ± 132* | ||
| Metformin | 7 | 3171 ± 401 | 3179 ± 390 | 10730 ± 582 | 10670 ± 561 | ||
| Propranolol | 6 | 11.8 ± 3.7 | 5.8 ± 3.6 | 201 ± 24 | 89 ± 17** | ||
↵* p < 0.05; ** p < 0.01; *** p < 0.0001 vs. OCT wild-type.