Enzyme | MAP | PEITC | Coumarin | ||||||
---|---|---|---|---|---|---|---|---|---|
KD | KD/KD (2A13 wt.) | KD | KD/KD (2A13 wt.) | KD | KD/KD (2A13 wt.) | ||||
μM | μM | μM | |||||||
CYP2A6 | 74 | 74 | 6.1 | 14 | 3.1 | 1.1 | |||
CYP2A13 | 1.0 | 1.0 | 0.43 | 1.0 | 2.7 | 1.0 | |||
CYP2A13 mutants | |||||||||
L110V | 1.6 | 1.6 | 0.46 | 1.1 | 1.7 | 0.63 | |||
A117V | 4.1 | 4.1 | 1.2 | 2.8 | 1.8 | 0.67 | |||
S208I | 12.0 | 12. | 1.4 | 3.3 | 7.9 | 2.9 | |||
A213S | 2.9 | 2.9 | 0.87 | 2.0 | 3.4 | 1.3 | |||
F300I | 15.0 | 15 | 6.0 | 14. | 14.0 | 5.2 | |||
A301G | 8.2 | 8.2 | 3.1 | 7.2 | 4.2 | 1.6 | |||
M365V | 1.7 | 1.7 | 1.7 | 4.0 | 5.0 | 1.9 | |||
L366I | 0.56 | 0.56 | 0.34 | 0.79 | 0.57 | 0.21 | |||
G369S | 1.6 | 1.9 | 1.8 | 4.2 | 6.0 | 2.2 | |||
H372R | 0.77 | 0.77 | 1.0 | 2.3 | 1.5 | 0.56 | |||
CYP2A6 mutants | |||||||||
I208S/I300F/G301 | 12.0 | 12. | 4.9 | 11 | 6.0 | 2.2 | |||
I300F/G301A/S369G | 12.3 | 12 | 1.9 | 4.4 | 3.1 | 1.1 | |||
I208S/I300F/G301A/S369G | 3.5 |
3.5
| 2.1 |
4.8
| 2.3 |
0.85
|