Primers used for the amplification and sequencing for the CYP2D6 gene
Primer Name | Orientation | Site | Sequence (5′ to 3′) | Position in Reference Sequencea |
|---|---|---|---|---|
| CYP505F | Forward | Amplification of CYP2D6 gene | CACTGGCTCCAAGCATGGCAG | 13044-13064b |
| 3′2D6R | Reverse | ACTGAGCCCTGGGAGGTAGGTA | 6350-6371c | |
| Sequencing primers | ||||
| CYP507 | Forward | Promoter | AACGTTCCCA CCAGATTTC | 561-579 |
| CYP509 | Forward | Promoter | GTAAGT GCCAGTGACA GATAAG | 1115-1136 |
| 2d6-11 | Reverse | Promoter | AGGATCCTTTGTTCAGGATATGTTGC | 531-556 |
| 2d6-12 | Reverse | Promoter | CACCAAGTACCCCACTTCCC | 811-820 |
| 2d6-1 | Forward | Exon 1 | CATGTGGACTTCCAGAACACACC | 1761-1783 |
| 2d6-2 | Forward | Intron 1 | GGTTCAAACCTTTTGCACTG | 2179-2198 |
| 2d6-3 | Forward | Exon 2 | GTCGTGCTCAATGGGCTG | 2557-2574 |
| 2d6-4 | Forward | Intron 2 | AAGGTGGATGCACAAAGAGT | 3077-3096 |
| 2d6-5 | Forward | Exon 4 | GACCTAGCTCAG GAGGGACT | 3579-3596 |
| 2d6-6 | Forward | Exon 5 | AGCTGGATGAGCTGCTAACT | 4142-4161 |
| 2d6-7 | Forward | Intron 6 | CCTGACCTCCTCCAACATAG | 4686-4705 |
| 2d6-8 | Forward | Intron 7 | CACCTAGTCCTCAATGCCAC | 5062-5080 |
| 2d6-9 | Forward | Intron 8 | GAGTCTTGCAGGGGTATCAC | 5598-5617 |
↵ a Position in the reference sequence, M33388.
↵ b The forward primer is the same as that in the previous report (Lovlie et al., 1996).
↵ c The reverse primer for the amplification of CYP2D6 gene was described previously (Gaedigk et al., 1999).