TABLE 4

Transcriptional and translational regulation of ADH3

Data sources: Kwon et al., 1999, 2001.

Transcription
Translationa
-20Tsp
Tsp+10b
 Expression
uORF1
 uORF2
 ORF (ADH3)
Expression
Sp1 Sp1 Baseline AUG AUG AUG Baseline
Sp1 Sp3/4 ↓ CUG AUG AUG ↑
Sp3/4 Sp1 ↓ AUG CUG AUG ↑
Sp3/4
Sp3/4
↓ ↓
CUG
CUG
AUG
↑

  • Tsp, transcriptional start point; AUG, translational start codon; CUG, mutated start codon; Sp, specificity protein transcription factor.

  • ↵ a The top row reflects the two weak translational start sites upstream of the ADH3 open reading frame (ORF). The lower rows reflect site-directed mutations in these uORFs.

  • ↵ b A polymorphism in this region has also been shown to decrease ADH3 expression in vitro (Hedberg et al., 2001).