Sequence of primers used for amplification of UGT cDNAs
Isoform | Primer | Sequence | Positiona |
|---|---|---|---|
| UGT1A3 | 1A3-Sb | 5′-ATG GCA ATG TTG AAC AAT ATG T-3′ | 340-361 |
| 1A ex5-ASc | 5′-GCA CTC TGG GGC TGA TTA AT-3′ | 1720-1739 | |
| UGT1A5 | 1A5-Sb | 5′-ACA ATA TGT CTT TGA TCA TA-3′ | 353-372 |
| 1A ex5-ASc | 5′-GCA CTC TGG GGC TGA TTA AT-3′ | 1720-1739 | |
| UGT1A7 | 1A7 ex1-S | 5′-GAA GTT CTC TGA TGG CTC GT-3′ | -10-9 |
| 1A ex5-ASc | 5′-GCA CTC TGG GGC TGA TTA AT-3′ | 1708-1727 | |
| UGT1A8 | 1A8 ex1-S | 5′-CCC ATT CCC CTA TGT GTT TC-3′ | 498-518 |
| 1A ex5-ASc | 5′-GCA CTC TGG GGC TGA TTA AT-3′ | 1708-1727 | |
| UGT1A10 | 1A10 ex1-S | 5′-CCC AGC TGC TGG CTC G-3′ | 557-578 |
| 1A ex5-ASc | 5′-GCA CTC TGG GGC TGA TTA AT-3′ | 1708-1727 | |
| UGT2B4 | 2B4-Sb | 5′-CAT CTT CAG CTT CCA TTT C-3′ | 170-188 |
| 2B4 ex6-AS | 5′-TAT CTG GTT TTC CAG CTT C-3′ | 1603-1621 | |
| UGT2B7 | 2B7 ex1-S | 5′-ATT GCA CCA GGA TGT CTG-3′ | -10-7 |
| 2B7 ex6-AS | 5′-CTT GCA TCA CAA TCT TTC TTG CTG-3′ | 1648-1671 | |
| UGT2B10 | 2B10 ex1-S | 5′-GGC TCT GAA ATG GAC TA-3′ | -32 to -19 |
| 2B10 ex6-AS | 5′-TGA TGA TAA ATA GCA CG-3′ | 1506-1522 | |
| UGT2B11 | 2B11-Sb | 5′-CTT CCA TTC TTT TTG ATC CCA ATG AT-3′ | 179-204 |
| 2B11 ex6-AS | 5′-AGC AAT GTT ATC AGG TTG ATC AA-3′ | 1190-1212 | |
| UGT2B15 | 2B15 ex1-S | 5′-GAA AAG AAG CAT TGC ATA AG-3′ | -25 to -6 |
| 2B15 ex6-AS | 5′-GAG GAG TCC CAT CTT TCA-3′ | 1622-1639 | |
| UGT2B17 | 2B17 ex1-S | 5′-GAA AAG AAG CAT TGC ATA AG-3′ | -25 to -6 |
| 2B17 ex6-AS | 5′-GAG GAG TCC CAT CTT TTG-3′ | 1622-1639 | |
| UGT2B28 | 2B28-Sa | 5′-ATC CCA ATG ACG CAT TCA CTC TTA AAC TC-3′ | 194-222 |
|
| 2B28 ex6-AS | 5′-AAT GTT ATC AGG TTG ATC CCA-3′ | 1189-1209 |