Sequence of primers used for real-time RT-PCR analyses
Isoforms | Primer | Sequence | Positiona | Annealing Temperature (°C) |
|---|---|---|---|---|
| UGT1A1 | 1A1-Sb | 5′-CCT TGC CTC AGA ATT CCT TC-3′ | 696-715 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 907-929 | ||
| UGT1A3 | 1A3-S1 | 5′-GTT GAA CAA TAT GTC TTT GGT CT-3′ | 348-370 | 58 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 910-932 | ||
| UGT1A4 | 1A4-S1 | 5′-CCT GCT GTG TTT TTT TGG AGG T-3′ | 502-523 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 910-932 | ||
| UGT1A5 | 1A5-S1 | 5′-TGT CCT ACC TTT GCC ATG CTG-3′ | 659-679 | 58 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 910-932 | ||
| UGT1A6 | 1A6-S1 | 5′-CAA CTG TAA GAA GAG GAA AGA C-3′ | 831-851 | 58 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 910-932 | ||
| UGT1A7 | 1A7-S1 | 5′-CCC CTA TTT TTT CAA AAA TGT CTT-3′ | 660-683 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT1A8 | 1A8-S1 | 5′-GGT CTT CGC CAG GGG AAT AG-3′ | 498-517 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT1A9 | 1A9-S1 | 5′-GAA CAT TTA TTA TGC CAC CG-3′ | 646-665 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT1A10 | 1A10-Sb | 5′-CTC TTT CCT ATG TCC CCA ATG A-3′ | 557-578 | 64 |
| 1A ex2-ASb | 5′-ATT GAT CCC AAA GAG AAA ACC AC-3′ | 898-920 | ||
| UGT2B4 | 2B4-S1 | 5′-TGG TGA GCT GCT GGC CGA GT-3′ | 468-487 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 729-750 | ||
| UGT2B7 | 2B7-S1 | 5′-GGA AAT CAT GTC AAT ATT TGG-3′ | 339-359 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 729-750 | ||
| UGT2B10 | 2B10-Sc | 5′-TGA CAT CGT TTT TGC AGA TGC TTA-3′ | 432-455 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 726-747 | ||
| UGT2B11 | 2B11-S1 | 5′-AGA AAT CCT GTG GGA ATT AT-3′ | 339-358 | 54 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 729-750 | ||
| UGT2B15 | 2B15-Sc | 5′-GTG TTG GGA ATA TTA TGA CTA CAG TAA C-3′ | 348-375 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 732-753 | ||
| UGT2B17 | 2B17-Sc | 5′-GTG TTG GGA ATA TTC TGA CTA TAA TAT A-3′ | 348-375 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 732-753 | ||
| UGT2B28 | 2B28-S1 | 5′-ACC GTT TGT GTA CAG TCT CT-3′ | 498-517 | 58 |
| 2B ex2-AS | 5′-CAT TGT CTC AAA TAA TGT AGT G-3′ | 729-750 | ||
| GAPDH | GAPDH-Sb | 5′-CCA GGG CTG CTT TTA ACT C-3′ | 56-74 | 68 |
|
| GAPDH-ASb | 5′-GCT CCC CCC TGC AAA TGA-3′ | 330-347 |
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