Comparison of lithocholic acid metabolite formation with testosterone 6β-hydroxylase activity in four human liver microsome samples
Details of the human liver donors are as follows: HH13, Asian male, 55 years of age; HG95, Hispanic female, 47 years of age; HH18, African American female, 78 years of age; and HH837, Asian female, 52 years of age. Testosterone 6β-hydroxylase activities were determined by BD Biosciences. The r2 value is the square of the correlation coefficient (i.e., coefficient of determination) determined for testosterone 6β-hydroxylase activities and rates of metabolite formation of individual lithocholic acid metabolites of the four human liver samples.
Sample | Testosterone 6β-Hydroxylase Activity | Rates of Lithocholic Acid Metabolite Formation | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 3-Ketocholanoic Acid | Hyodeoxycholic Acid | Ursodeoxycholic Acid | Murideoxycholic Acid | 6-Ketolithocholic Acid | ||||||
| pmol/min/mg protein | pmol/min/mg protein | pmol/min/mg protein | pmol/min/mg protein | pmol/min/mg protein | pmol/min/mg protein | |||||
| HH13 | 890 | 149.3 ± 9.1 | 11.3 ± 0.2 | 4.6 ± 0.2 | 2.5 ± 0.1 | 0.9 ± 0.0 | ||||
| HG95 | 650 | 54.4 ± 5.6 | 1.6 ± 0.4 | 8.9 ± 1.34 | 0.7 ± 0.1 | 1.7 ± 0.5 | ||||
| HH18 | 12,000 | 542.4 ± 9.3 | 118.0 ± 3.4 | 35.1 ± 1.6 | 11.2 ± 0.5 | 10.6 ± 0.4 | ||||
| HH837 | 13,700 | 750.1 ± 38.3 | 160.5 ± 2.2 | 47.3 ± 1.0 | 15.4 ± 0.3 | 16.4 ± 0.5 | ||||
| r2 Value |
| 0.96 | 0.98 | 0.97 | 0.97 | 0.95 | ||||