Rat hepatic microsomes | | | | |
Vmax [pmol/(min · mg) protein] | 118.2 ± 8.9 | 82.1 ± 7.5 | 83.9 ± 4.3 | 61.7 ± 2.2 |
Km (μM) | 17.5 ± 0.6 | 19.3 ± 1.0 | 35.4 ± 1.8 | 46.0 ± 1.2 |
Vmax/Km ratio [μl/(min · mg) protein] | 6.8 ± 0.7 | 4.3 ± 0.5 | 2.4 ± 0.2 | 1.3 ± 0.1 |
Human hepatic microsomes | | | | |
Vmax [pmol/(min · mg) protein] | 116.1 ± 6.5 | 85.1 ± 2.3 | 67.1 ± 4.5 | 87.9 ± 9.5 |
Km (μM) | 16.8 ± 1.4 | 17.9 ± 1.0 | 34.8 ± 2.9 | 42.8 ± 4.5 |
Vmax/Km ratio [μl/(min · mg) protein] | 6.9 ± 0.3 | 4.8 ± 0.2 | 1.9 ± 0.1 | 2.1 ± 0.1 |
CYP3A4 isoform | | | | |
Vmax [pmol/(min · mg) protein] | 92.6 | 59.2 | 62.1 | 59.5 |
Km (μM) | 15.0 | 14.2 | 30.2 | 44.6 |
Vmax/Km ratio [μl/(min · mg) protein] | 6.2 | 4.2 | 2.1 | 1.3 |