CYP450 Isoformsa | Metabolic Rate | |||||||
---|---|---|---|---|---|---|---|---|
PPT | Rh1 | Rg2 | Rf | |||||
2 μM | 20 μM | 5 μM | 50 μM | 5 μM | 50 μM | 5 μM | 50 μM | |
pmol/(min · pmol) protein | ||||||||
3A4 | 3.99 | 33.77 | 6.36 | 27.91 | 4.68 | 25.39 | 3.01 | 23.99 |
3A5 | 0.19 | 0.71 | 0.19 | 0.71 | 0.3 | 2.02 | 0.18 | 1.01 |
↵a A panel of 10 recombinant human P450 isoforms was screened, and only CYP3A4 and, to a very minor extent, CYP3A5 were found to be active toward the oxygenation metabolism of PPT-type ginsenosides.