TABLE 1

Comparison of 96-well plates with various types of basement membrane on CYP3A induction. Hepatocytes (lot LOF) were cultured on a 96-well plate coated with two lots of TL Matrigel, collagen type I, collagen type IV, laminin, fibronectin, PLO/LM, or PDL. Hepatocytes cultures were treated with 0.1% dimethyl sulfoxide (control), 10 μM RIF, 30 μM phenytoin, or 30 μM carbamazepine, and determined the induced CYP3A activity. CYP3A induction responses, determined by 6β-hydroxytestosterone formation, are represented as CYP3A activity [pmol/(h · million cells)], fold induction, or %RIF. Data represent mean ± S.D. calculated from four separate wells.

TL Matrigel lot 14340TL Matrigel lot 92069Collagen Type ICollagen Type IVLamininFibronectinPLO/LMPDL
Activity [pmol/(h · million cells)]
    Control994 ± 1521557 ± 450250 ± 12307 ± 35336 ± 60235 ± 9193 ± 18351 ± 21
    10 μM Rifampicin15699 ± 185625412 ± 23333940 ± 6285644 ± 1985522 ± 10383286 ± 5231962 ± 5999319 ± 908
    30 μM Phenytoin6507 ± 8739396 ± 266637 ± 74897 ± 73936 ± 212423 ± 102336 ± 741427 ± 250
    30 μM Carbamazepine4260 ± 5357070 ± 487532 ± 93732 ± 831234 ± 106442 ± 113420 ± 761315 ± 80
Fold
    Control11111111
    10 μM Rifampicin15.8 ± 3.116.3 ± 515.8 ± 2.618.4 ± 2.216.4 ± 4.314 ± 2.310.2 ± 3.326.6 ± 3
    30 μM Phenytoin6.5 ± 1.36 ± 1.82.5 ± 0.32.9 ± 0.42.8 ± 0.81.8 ± 0.41.7 ± 0.44.1 ± 0.8
    30 μM Carbamazepine4.3 ± 0.84.5 ± 1.42.1 ± 0.42.4 ± 0.43.7 ± 0.71.9 ± 0.52.2 ± 0.43.7 ± 0.3
%RIF
    Control00000000
    10 μM Rifampicin100100100100100100100100
    30 μM Phenytoin37.5 ± 7.732.9 ± 3.910.5 ± 2.711.1 ± 1.611.6 ± 4.86.2 ± 3.58.1 ± 5.112.0 ± 3.0
    30 μM Carbamazepine22.2 ± 4.723.1 ± 3.67.7 ± 2.98 ± 1.717.3 ± 4.26.8 ± 3.912.9 ± 6.210.8 ± 1.4