TABLE 1

Reduction of N-hydroxy-sulfonamides

A complete incubation mixture consisted of 200 pmol of cytochrome b₅, 0.05 units of NADH cytochrome b₅ reductase, and 2.4 to 3 μg of native mARC (purified from porcine liver mitochondria) or 10 to 30 μg of mARC-1 or mARC-2 (human, recombinantly expressed), 0.5 mM N-hydroxyvaldecoxib, or 2 mM NOHBSA, respectively, NOHTSA, and 1.0 mM NADH in a total volume of 150 μl of potassium phosphate buffer (100 mM). Sample preparation and HPLC analysis were described under Materials and Methods. Data are means ± S.D. of two determinations.