TABLE 2

Kinetic parameters of the reduction of N-hydroxy-sulfonamides to sulfonamide

substrateParametersmARC-1mARC-2
N-Hydroxy-valdecoxibKma0.50.4
Vmaxb4.61.3
Efficiencyc9.23.3
NOHBSAKma1.01.9
Vmaxb6.48.1
Efficiencyc6.44.3
  • a mM.

  • b nmol/(min · mg total protein).

  • c Efficiency of catalysis (Vmax/Km).