Overview of characteristics of liraglutide degradation products obtained after liraglutide incubation with DPP-IV and NEP in vitro
The degradation products are named by a prefix for the enzyme involved, D for DPP-IV and N for NEP, and the number of amino acids residues.
| NEP | DDP-IV | Molecular Weight (Monoisotopic Mass) | RtR | |
|---|---|---|---|---|
| Liraglutide(7–37) | 3749.0 | 1.00 | ||
| Degradation product | ||||
| D1 (9–37) | ✓ | 3540.8 | 1.06 | |
| N1 (20–27) | ✓ | 1211.7 | 1.03 | |
| N2 (20–37) | ✓ | 2367.3 | 1.04 | |
| N2 (19–27) | ✓ | 1374.8 | 1.04 | |
| N3 (19–37) | ✓ | 2530.4 | 1.07 | |
| N3 (24–27) | ✓ | 784.5 | 1.07 | |
| N4 (25–27) | ✓ | 713.5 | 1.09 | |
| N5 (26–27) | ✓ | 642.4 | 1.11 | |
| N5 (20–28) | ✓ | 1358.7 | 1.11 | |
| N6 (25–26) | ✓ | 584.4 | 1.13 | |
| N7 (24–28) | ✓ | 931.6 | 1.15 | |
| N8 (25–28) | ✓ | 860.5 | 1.17 |