Enzyme | Substrate | Metabolite | Mass Transition (m/z) | Mode | CE | DP | Linear Range |
---|---|---|---|---|---|---|---|
eV | eV | ||||||
CYP1A2 | Phenacetin (10 μM) | Acetaminophen | 152.13 > 110.15 | ESI+ | 22 | 46 | 10 nM–10 μM |
CYP2C9 | Diclofenac (5 μM) | 4′-Hydroxydiclofenac | 312.09 > 230.01 | ESI+ | 45 | 41 | 1 nM–1 μM |
CYP2C19 | (S)-Mephenytoin (50 μM) | 4′-Hydroxymephenytoin | 235.10 > 150.21 | ESI+ | 24 | 56 | 1 nM–1 μM |
CYP2D6 | Dextromethorphan (5 μM) | Dextrorphan | 258.17 > 157.10 | ESI+ | 51 | 81 | 1 nM–1 μM |
CYP3A4 | Midazolam (1 μM) | 1′-Hydroxymidazolam | 341.82 > 203.20 | ESI+ | 38 | 71 | 1 nM–1 μM |
CE, collision energy; DP, declustering potential; ESI, electrospray ionization.