Mean enzyme kinetic parameters for glucuronide conjugate formation from 1′- and 4-hydroxymidazolam by recombinant human UGT isoforms
Each data set is the average of triplicate incubations.
| UGT Isoform | Metabolite | Kinetic Parameters | |||
|---|---|---|---|---|---|
| Km | Vmax | Hill Coefficient (n) | CLint (Vmax/Km) | ||
| μM | pmol/(min · mg protein) | μl/(min · mg protein) | |||
| 1′-Hydroxymidazolam | |||||
| 1A4 | N-Glucuronide | 15.9 | 468.3 | 1.31 | 29.5 |
| 2B4 | O-Glucuronide | 29.8 | 127.0 | 1.00 | 4.3 |
| 2B7 | 35.5 | 90.1 | 0.83 | 2.5 | |
| 4-Hydroxymidazolam | |||||
| 1A4 | Glucuronide I | 40.8 | 1064.2 | 1.23 | 26.1 |