Covalent binding of [14C]elzasonan (10 μM) to liver microsomes under various conditions
Data are presented as means ± S.D. Means represent three replicates.
| Microsomes | Condition | Covalent Binding |
|---|---|---|
| pmol [14C]elzasonan Eq bound/mg microsomal protein | ||
| Human | − NADPH | 6.96 ± 0.39 |
| + NADPH | 191 ± 10 | |
| + NADPH, + GSH (5 mM) | 91.0 ± 10.6 | |
| + NADPH, + KCN (0.1 mM) | 165 ± 29 | |
| + NADPH, + lysine (1.0 mM) | 185 ± 16 | |
| + NADPH, + ketoconazole (1.0 μM) | 27.5 ± 2.7 | |
| + NADPH, + cytosol (3 mg/ml) | 176 ± 47 | |
| + NADPH, acid precipitation | 54.8 ± 6.7 | |
| Rat | + NADPH | 33.6 ± 7.8 |
| Dog | + NADPH | 95.6 ± 13.9 |