Kinetic parameters of MMF hydrolysis in human tissue fractions
Values are represented as mean (S.D.).
| Vmax | Km | Clint (Vmax/Km) | |
|---|---|---|---|
| nmol/(min · mg protein) | μM | nl/(min · mg protein) | |
| HIM | |||
| High affinity | 15.6 (3.0) | 27 (9) | 571 |
| Low affinity | 19.7 (2.4) | 782 (512) | 25 |
| HJC | |||
| High affinity | 4.6 (1.4) | 15 (10) | 307 |
| Low affinity | 14.2 (3.6) | 1091 (432) | 13 |
| HIC | |||
| High affinity | 5.9 (1.7) | 18 (12) | 339 |
| Low affinity | 12.0 (8.2) | 1656 (2710) | 7 |
| HLM | 3073 (224) | 992 (138) | 3098 |
| HLMa | 1358 (58) | 1038 (86) | 1308 |
| HLC | 75 (4) | 329 (40) | 230 |
↵a Kinetic parameters were obtained from our previous report (Fujiyama et al, 2009).