Enzyme | Detergenta | kcat | Km | kcat/Km |
---|---|---|---|---|
min−1 | mM | min−1 mM−1 | ||
MBP-FMO3 | Minimal detergent | 10.8 ± 0.2 | 32 ± 2 | 0.34 ± 0.01 |
CHAPS | 11.5 ± 0.4 | 38 ± 3 | 0.30 ± 0.02 | |
Triton X-100 | 13.3 ± 0.7 | 15 ± 2 | 0.86 ± 0.07b | |
Supersome FMO3 | Minimal detergent | 33 ± 0.3 | 35 ± 2c | 0.91 ± 0.07 |
CHAPS | 40 ± 2c | N.D. | N.D. | |
Triton X-100 | 43 ± 6c | 25 ± 3c | 1.6 ± 0.1c,d |
N.D., not determined.
↵a When present, detergent concentrations were 0.5% (w/v). Unless otherwise indicated, values are the fitting value and error from a single determination.
↵b Comparison of this value to that obtained with minimal detergent conditions yielded p = 1.3 × 10−3.
↵c Indicated values are the average and S.D. of two or more independent determinations. When present, detergent concentrations were 0.5% (w/v).
↵d Comparison of this value to that obtained with minimal detergent conditions yielded p < 1 × 10−3.