TABLE 1

The effect of 24-h exposure to nanosilver on cell viability

Plated cells were exposed to nanosilver-containing medium for 24 h and evaluated for viability with a MultiTox-Fluor Multiplex Cytotoxicity assay (Promega). Values are means ± S.E.M. for quadruplicate wells. Viability was expressed relative to cells exposed to nanosilver-free medium controls. Derived LD₅₀ values were determined by interpolation.

Nanosilver	Cell Viability^{^a}
Nanosilver	MDR1.C	DPX2	1A2DRE
μg/ml	(%) mean ± S.E.M.
0	100 ± 1.2	100 ± 1.3	100 ± 1.4
1	113 ± 4.7	98 ± 1.2	105 ± 4.0
2.5	95 ± 1.9	91 ± 2.0^{^b}	75 ± 6.2^{^b}
5	52 ± 8.9^{^b}	24 ± 0.2^{^b}	23 ± 1.9^{^b}
6	24 ± 1.7^{^b}	28 ± 0.2^{^b}	15 ± 1.9^{^b}
7	18 ± 5.6^{^b}	26 ± 0.4^{^b}	27 ± 1.9^{^b}
10	5 ± 0.3^{^b}	24 ± 0.4^{^b}	10 ± 0.7^{^b}
Derived LD₅₀	5.1 μg/ml	4.0 μg/ml	3.7 μg/ml

↵a Statistically significant (p < 0.05) changes from controls.
↵b Derived LD₅₀ values are 5.1, 4.0, and 3.7 μg/ml for MDR1.C, DPX2, and 1A2DRE, respectively.