LC gradients used for HPLC and LC/MS analysis
| Gradient A for Analysis of In Vitro Samples | Gradient B for Analysis of Rat and Dog Samples | Gradient C for Analysis of Mouse, Monkey, and Human Samples | ||||||
|---|---|---|---|---|---|---|---|---|
| Time (min) | A (%) | B (%) | Time (min) | A (%) | B (%) | Time (min) | A (%) | B (%) |
| 0 | 90 | 10 | 0 | 90 | 10 | 0 | 95 | 5 |
| 3 | 90 | 10 | 6 | 90 | 10 | 3.0 | 95 | 5 |
| 25 | 60 | 40 | 35 | 60 | 40 | 3.1 | 90 | 10 |
| 45 | 15 | 85 | 65 | 15 | 85 | 13.0 | 90 | 10 |
| 50 | 15 | 85 | 25 | 80 | 20 | |||
| 50 | 70 | 30 | ||||||
| 51 | 50 | 50 | ||||||
| 70 | 10 | 90 | ||||||
| 80 | 10 | 90 | ||||||