TABLE 2

Kinetic parameters (mean estimate ± S.E.) of BUP glucuronidation in pools of HLMs genotyped for UGT1A1*28 and UGT2B7 G–842A

Pool of Genotyped HLMs	V_max	S₅₀^a	Cl_max^b	n^a
	pmol/mg protein/min	μM	μl/min/mg protein
UGT1A11/1
GG–842 (n = 5)	1689.6 ± 39.9	11.2 ± 0.6	75.2	1.9 ± 0.2
G–842A (n = 10)	2725.0 ± 131.1	10.6 ± 1.1	134.4	2.9 ± 0.8
−842AA (n = 6)	2817.0 ± 131.2	10.6 ± 1.1	136.2	2.6 ± 0.6
Mixed results^c	2400.3 ± 89.4	10.7 ± 0.8	115.2	2.5 ± 0.5
UGT1A128/28 (n = 7)	1726.7 ± 69.6	9.5 ± 0.8	91.4	2.2 ± 0.4

↵a S₅₀ and n (Hill coefficient) computed by Hill equation.
↵b Cl_max calculated as described under Material and Methods.
↵c Computed from the average of the velocities observed in the UGT2B7-genotyped pools.