Incubation Time | K | Vmax | Vmax/Km | Enzyme Abundances* | CLint, expressed P450 | fmP450 % | |
---|---|---|---|---|---|---|---|
min | μM | pmol/pmol P450/min | μl/pmol P450/min | pmol P450/mg protein | μl/mg protein/min | ||
CYP1A2 | 1 | 1.58 ± 1.35 | 2.27 ± 0.46 | 1.44 | 52 | 74.9 | 35.8 |
CYP2B6 | 1 | 2.08 ± 0.73 | 7.66 ± 0.69 | 3.68 | 11 | 40.5 | 19.4 |
CYP2C19 | 1 | 1.12 ± 0.25 | 7.52 ± 0.36 | 6.71 | 14 | 93.9 | 44.9 |
↵* The abundances of CYP1A2, CYP2B6, and CYP2C19 were obtained from the reported data in Rowland Yeo et al. (2004).