TABLE 1

Relative abundance of BMS-665053 and its metabolites, estimated by UV peak areas at 245 to 255 nm, in human and rat liver microsomal incubations in the presence of NADPH alone or NADPH and GSH at 37° C for 30 min

HPLC retention times of the parent drug and its metabolites are shown. Sample preparation, analysis, and data processing are described under Materials and Methods.

Parent Drug or Metabolite	HPLC Retention Time	Relative Abundance
		+NADPH		+NADPH + GSH
		HLMs	RLMs	HLMs	RLMs
	Min	%
BMS-665053	19.0	88	76	85	74
Met1	14.9	5	13	4	8
Met2	16.5	4	7	6	8
Met3	18.1	2	2	2	4
Met4	16.7	1	2	1	1
Met5	14.3	N.A.	N.A.	<1	1
Met6	13.5	N.A.	N.A.	<1	1
Met7	13.6	N.A.	N.A.	<1	1
Met8	15.6	N.A.	N.A.	1	2

N.A., not applicable.