TABLE 2

The genetic variants identified in cynomolgus and rhesus CYP3A5

Exon	Nucleotide Change^a	Amino Acid Change	Nucleotide Position^b	Number of Alleles^c			Site	Nucleotide Substitutions in CYP3A5 cDNA^d
				Cynomolgus		Rhesus		Nucleotide Substitutions in CYP3A5 cDNA^d
				Indochina	Indonesia	Rhesus		Human	Rhesus
1	ACAGT(C>A)CAGCA		−66	0/76	0/78	1/68	5′-UTR
	GCAAA(C>T)AGCAG		−58	3/76	0/78	0/68	5′-UTR
	AGACT(C>T)AGAGG		−31	2/76	0/78	0/68	5′-UTR
	AGGAG(A>G)CAGTT		−23	1/76	0/78	1/68	5′-UTR
	GTGGC(G>A)ATGGA		−1	4/76	0/78	0/68	5′-UTR
	AGCCT(G>A)GTGCT		57	1/76	0/78	0/68
2	CTATA(C>T)GGGAC		75	9/76	0/80	0/68		T
3	CAGGG(T>G)CTCTG		168	0/76	0/80	8/68
	TGTTA(T>C)AAAAA		195	0/76	9/80	0/68
	TATGG(A>G)AAAAT		207	3/76	0/80	66/68			G
4	GATCC(C>T)GACAT		261	14/76	11/80	3/68
	CCCGA(C>G)ATGAT	D88E	264	3/76	0/80	68/68			G
5	AGAAT(T>A)CGGTC		387	64/76	80/80	66/66		A	A
	TTGCT(G>C)TCTCC		399	1/76	0/80	21/66			C
	ACCAG(C>T)GGAAA		417	1/76	1/80	0/66
	TCAAG(G>A)AGATG	E144K	430	0/76	5/80	0/66
6	GAGAT(A>G)TGTTG	M155V	463	2/76	0/80	38/68		G
	ATATG(T>C)TGGTG		466	0/76	0/80	2/68
	GGCAA(G>A)CCTGT		504	0/76	0/80	2/68
7	TTAAG(A>T)AGTTC	K209X	625	0/76	3/516^e	0/68	SRS2
	CTCTT(A>G)ACAAT		666	0/76	0/80	1/68
8	CCTTA(T>C)CCCAG	T230I	689	3/76	0/80	68/68		C	C
10	TGATC(A>T)GGAGC	Q293L	878	0/76	1/80	0/68		T
	TATGA(A>G)CTGGC		960	0/76	0/80	2/68
	TGGCC(A>T)CTCAC	T323S	967	0/76	1/80	0/68
	AGAAA(C>T)TGCAG		991	0/76	1/80	0/68
11	GAGCC(T>C)GAGGA		1233	1/76	0/80	2/68
	CCTGA(G>A)GAGTT		1236	1/76	1/80	0/68
	GTTCC(G>A)CCCTG	R415H	1244	0/76	3/80	0/68
12	TGGAA(G>C)TGGAC	T437S	1310	66/76	80/80	68/68	Heme	C	C
13	TCCCC(T>C)TGAAA		1423	0/76	3/80	0/68
	GGCAA(C>G)CAAGG	N479K	1437	0/76	5/80	0/68	SRS6	G
	GAGTC(A>T)AGAGA		1485	1/76	0/80	0/68
	AGAGA(T>C)GGAAC		1491	1/76	0/80	0/68

↵a Nucleotide changes are indicated as a comparison to the reference sequence of cynomolgus CYP3A5 cDNA (GenBank accession number DQ074795).
↵b Position number is based on the first nucleotide of the initiation codon being +1. The upstream region is represented in negative numbers.
↵c CYP3A5 genotypes were determined for 78 cynomolgus (38 from Indochina and 40 from Indonesia) and 34 rhesus macaques.
↵d The CYP3A5 nucleotides different from cynomolgus macaque are shown for human (GenBank accession number NM_000777) and rhesus macaque (GenBank accession number NM_001040219). Any genetic polymorphisms of human CYP3A5 corresponding to macaque CYP3A5 alleles identified in this study were not reported to the human P450 allele nomenclature (http://www.cypalleles.ki.se/).
↵e For c.625A>T, 258 animals were used to calculate allele frequency in Indonesian population.