Primer sets used for introduction of restriction enzyme sites by mutagenesis: introduction of restriction site accompanied nucleotide substitutions that were corrected
| Introduction | Correction | |
|---|---|---|
| BsrGI site in rabbit AOX1 cDNA | ||
| Forward | 5′-cgagaaggtccgagccataaacttgtacaaggaaattg-3′ | 5′-cgagaaggtccgagccataaacttttacaaggaaattg-3′ |
| Reverse | 5′-caatttccttgtacaagtttatggctcggaccttctcg-3′ | 5′-caatttccttgtaaaagtttatggctcggaccttctcg-3′ |
| MluI site in monkey AOX1 cDNA | ||
| Forward | 5′-gctgtggaaaaattcaacgcgtagaattactggaagaagaaag-3′ | 5′-tgtggaaaaattcaacgcggagaattactggaagcagag-3′ |
| Reverse | 5′-ctttcttcttccagtaattctacgcgttgaatttttccacagc-3′ | 5′-ctctgcttccagtaattctccgcgttgaatttttccaca-3′ |
| MluI site in rabbit AOX1 cDNA | ||
| Forward | 5′-gaaagtggctgtggaaaagttcaacgcgtagaattactggaagcaga-3′ | 5′-ctgtggaaaagttcaacgcggagaattactggaagaagaaa-3′ |
| Reverse | 5′-tctgcttccagtaattctacgcgttgaacttttccacagccactttc-3′ | 5′-tttcttcttccagtaattctccgcgttgaacttttccacag-3′ |