Kinetic parameters for oxidation activities of cinchonidine and (S)-RS-8359 by mutant proteins of monkey AOX1
Eleven amino acids in the range of Ala997 to Ala1092 of monkey AOX1 were substituted with those of rabbit AOX1 with SDM. The number of substituted amino acids was single in mutants 1 to 4 and plural in mutants 5 to 10. Value is the mean ± S.D. of two experiments.
| Mutants | Cinchonidine | (S)-RS-8359 | |
|---|---|---|---|
| Activity | Km | Vmax | |
| nmol/min/mg protein | μM | nmol/min/mg protein | |
| 1 | N.D. | 36.6 ± 15.8 | 8.48 ± 5.71 |
| 2 | N.D. | 27.0 ± 3.13 | 7.20 ± 2.05 |
| 3 | N.D. | 26.4 ± 15.9 | 3.09 ± 0.892 |
| 4 | N.D. | 33.4 ± 7.01 | 6.98 ± 5.17 |
| 5 | N.D. | 36.0 ± 0.867 | 11.7 ± 0.333 |
| 6 | N.D. | 52.1 ± 8.00 | 2.71 ± 1.68 |
| 7 | N.D. | 96.5 ± 2.40 | 0.870 ± 0.307 |
| 8 | N.D. | 24.4 ± 1.53 | 3.95 ± 0.314 |
| 9 | N.D. | 15.7 ± 0.268 | 3.38 ± 0.173 |
| 10 | 148 ± 3.54 | 128 ± 10.8 | 13.2 ± 0.0930 |
N.D., activities were not detected.