Primer Name | Sequence |
---|---|
Cloning primers | Wild type |
CYP2C9_−735 fw | 5′-CAGACGCGTGCTATGAGCTGTGTGGC ACC |
CYP2C9_−421 fw | 5′-CAGACGCGTAATATACAAGG CATAGAATAT GG |
CYP2C9_−331 fw | 5′-CAGACGCGTCA GATTATTTACTTCAGTGCT |
CYP2C9 rev | 5′-CAGCTCGAGTGAAGCCTTCTCTTCTTGTTAA |
Mutant | |
CYP2C9_mut1_fw | 5′-CAAAGGACATTTTATTTTTTTTTGTATCAGTG |
CYP2C9_mut1_rev | 5′-AAA AATAAAATGTCCTTTGGTCTTGTTCT |
CYP2C9_mut2_fw | 5′-GACATTTTATTTTTATCTGTTTTAGTGGGTCAA |
CYP2C9_mut2_rev | 5′-ACAGATAAAAATAAAATGTCCTTTGGTCTT |
CYP2C9_mut3_fw | 5′-ATATAGTGGACCTAGGTTTTTGGTCAATTT |
CYP2C9_mut3_rev | 5′-ACCTAGGTCCACTATATGCTCCTTCTGAAA |
CYP2C9_mut4_fw | 5′-GGTGATTGGTCAATTAAAACATCAAAGAGG |
CYP2C9_mut4_rev | 5′-AATTGACCAATCACCTAGGTCCACTATATG |
CYP2C9_mut1+2_fw | 5′-CAAAGGACATTTTATTTTTTTTGTTTTTAGTG |
CYP2C9_mut1+2_rev | 5′-AAA AATAAAATGTCCTTTGGTCTTGTTCT |
EMSA oligonucleotides (forward) | |
2C19wt1+2 | 5′-ACCAAAGGACATTTTATTTTTATCTGTATCAGTGGGTCAAAGTCCTTTCA |
2C9wt3+4 | 5′-ATATAGTGGACCTAGGTGATTGGTCAATTTATCCATCAAAGAGGCACACA |
2C9mut1 | 5′-ACCAAAGGACATTTTATTTTTTTTTGTATCAGTGGGTCAAAGTCCTTTC |
2C9mut2 | 5′-ACCAAAGGACATTTTATTTTTATCTGTTTTAGTGGGTCAAAGTCCTTTCA |
2C9mut3 | 5′-ATATAGTGGACCTAGGTTTTGGTCAATTTATCCATCAAAGAGGCACACAC |
2C9mut4 | 5′-ATATAGTGGACCTAGGTGATTGGTCA ATTAAAACATCAAAGAGGCACACAC |
2C9mut1+2 | 5′-ACCA AAGGACATTT TATTTTTTTT TGTTTTAGTG GGTCAAAGTC CTTTCA |
Oligonucleotides used for ChIP | |
Primer set | 5′-CCAA CCAAGTACAG TGAAACT |
5′-TTAAGACAACCATGAGCTTGCACT |
fw, forward; rev, reverse primers.