TABLE 2

Kinetic parameters obtained by fitting various two-site models to kinetic data

Data are means (S.E.).

SubstrateModifierVmaxKsubKmodbcdKinetic ModelR2
pmol/min/mg proteinμMμM
t-ANDWithout modifier127 (19)33 (6.0)N.A.0.56 (0.12)N.A.N.A.Eq. 70.9695
TAMWithout modifier625 (22)1.4 (0.12)N.A.0.12 (0.02)N.A.N.A.Eq. 70.9712
DHTTAM9.8 (0.45)18 (2.2)0.35 (0.02)N.A8.4 (3.0)4.4 (2.1)Eq. 80.9911
TAMDHT562 (43)1.8 (0.24)58 (17)0.10 (0.04)0.52 (0.20)2.9 (1.9)Eq. 90.9743
TAMt-AND761 (51)1.3 (0.11)106 (21)0.18 (0.03)0.28 (0.12)1.2 (0.33)Eq. 100.9795
TAMt-AND761 (51)1.3 (0.11)106 (21)0.18 (0.03)0.14 (0.06)0.57 (0.17)Eq. 110.9795
  • N.A., not applicable. Ksub, binding affinity of the substrate; Kmod, binding affinity of the modifier.