TABLE 3

Kinetic parameters for DHT glucuronidation in the presence or absence of TAM

The Michaelis-Menten equation (eq. 2) was used to fit individual kinetic data sets. Data are means (S.E.). CLint equates to Vmax/Km; the TAM concentration was corrected for nonspecific binding.

TAMKmVmaxCLint
μMpmol/min/mg proteinμl/min/mg protein
017 (1.6)9.5 (0.3)0.55
0.1122 (2.5)15 (0.60)0.67
0.2329 (3.3)18 (0.79)0.63
0.4561 (4.9)27 (1.1)0.45
0.90185 (32)47 (5.7)0.25
1.80227 (89)47 (14)0.20