TABLE 1

Relative mRNA levels of P450s in human liver and WAT

Relative mRNA levels of P450 were measured using RT-PCR in uncultured (explants t = 0, n = 6) or cultured (explants t = 48 h, n = 6, and primary culture of adipocytes, n = 6) WAT and liver biopsies (pool, n = 12). Relative mRNA levels were calculated by dividing P450 levels by TBP levels and are means ± S.D. CYP2F1 and CYP2W1 mRNA were not detected and are therefore not shown in this table.

P450	Liver Biopsies	Subcutaneous WAT			Visceral WAT
P450	Liver Biopsies	Explants (t = 0)	Explants (t = 48 h)	Adipocytes	Explants (t = 0)	Explants (t = 48 h)
CYP1A1	24,432	571 ± 1080	8561 ± 6494	71 ± 59	222 ± 220	1839 ± 1258
CYP1A2	323,914	N.D.	N.D.	4 ± 2	N.D.	N.D.
CYP1B1	1884	1251 ± 564	8396 ± 3793	71,850 ± 14,605	9336 ± 2119	18,461 ± 5081
CYP2A6	144,272	N.D.	N.D.	N.D.	14 ± 19	N.D.
CYP2A7	28,956	N.D.	N.D.	N.D.	N.D.	N.D.
CYP2B6	49,353	50 ± 54	192 ± 304	19 ± 8	76 ± 42	19 ± 8
CYP2C18	54,865	219 ± 121	N.D.	N.D.	104 ± 98	9 ± 3
CYP2C19	74,445	60 ± 50	N.D.	N.D.	48 ± 40	7 ± 3
CYP2C8	360,803	41 ± 31	116 ± 139	23 ± 13	246 ± 286	46 ± 20
CYP2C9	494,491	9 ± 18	N.D.	N.D.	40 ± 36	N.D.
CYP2D6	86,014	145 ± 22	139 ± 64	120 ± 49	337 ± 99	166 ± 46
CYP2E1	3,244,323	155 ± 40	98 ± 25	105 ± 52	268 ± 202	136 ± 24
CYP2J2	50,999	17 ± 24	7 ± 5	5 ± 1	349 ± 304	78 ± 77
CYP2R1	457	133 ± 90	510 ± 186	265 ± 71	239 ± 83	505 ± 161
CYP2S1	895	201 ± 153	97 ± 54	7 ± 4	247 ± 95	148 ± 78
CYP2U1	2610	2135 ± 803	1750 ± 846	3599 ± 345	3567 ± 1041	1890 ± 539
CYP3A4	50,5805	116 ± 83	6 ± 6	N.D.	30 ± 11	2 ± 1
CYP3A43	5094	18 ± 14	N.D.	4 ± 1	14 ± 81	12 ± 1
CYP3A5	75,422	426 ± 358	199 ± 77	6 ± 4	350 ± 45	89 ± 36
CYP3A7	16,355	N.D.	N.D.	N.D.	30 ± 18	3 ± 7

N.D., not detected (Ct >33).