Quantities and volumes during vesicle preparation
| Protein | Cells (109)a | Lysate Volume | Crude Preparation Volume | Vesicle Preparation | |
|---|---|---|---|---|---|
| Volume | Protein | ||||
| ml | mg | ||||
| BCRP stable | 2.1 | 200 | 5 | 0.8 | 14 |
| MRP2 stable | 2.9 | 200 | 5 | 0.8 | 20 |
| MRP2 transient | 9.8 | 900 | 70 | 9.4 | 166 |
| BCRP transient | 10.0 | 1400 | 80 | 27.0 | 545 |
| P-gp transient | 8.0 | 1400 | 80 | 7.6 | 186 |
↵a Cell counts are amounts of cells (total count) used for vesicle preparation.