Kinetics of 17-OHPC metabolism
The data represent mean ± S.D.
| Metabolite Numbers | Vmax | Vmax | Km |
|---|---|---|---|
| nmol/min/million cells | nmol/min/pmol P450 | μM | |
| Fetal_M1 | 0.46 ± 0.16 | 12.3 ± 5.1 | |
| Fetal_M2 | 0.50 ± 0.11 | 11.7 ± 5.3 | |
| Fetal_M3 | 0.65 ± 0.10 | 25.2 ± 11.0 | |
| Adult_M2 | 1.3 ± 0.52 | 32.60 ± 49.4 | |
| Adult_M4 | 0.35 ± 0.23 | 33.10 ± 25.1 | |
| Adult_M5 | 0.79 ± 0.50 | 38.80 ± 25.7 | |
| CYP3A7_M1 | 0.0103 ± 0.002 | 1.8 ± 0.90 | |
| CYP3A7_M2 | 0.0104 ± 0.001 | 1.7 ± 0.50 | |
| CYP3A7_M3 | 0.0130 ± 0.002 | 5.2 ± 2.1 | |
| CYP3A4_M2 | 0.20 ± 0.02 | 57.5 ± 14.7 | |
| CYP3A4_M4 | 0.04 ± 0.006 | 40.1 ± 16.1 | |
| CYP3A4_M5 | 0.032 ± 0.003 | 10.5 ± 4.8 |
Fetal, fetal hepatocytes (n = 4); adult, adult hepatocytes (n = 4); CYP3A7, expressed CYP3A7 (n = 2); CYP3A4, expressed CYP3A4 (n = 2).