TABLE 1

Roles of P450s in TPV metabolic pathways

Duplicate incubations were conducted in 1× PBS (pH 7.4) containing TPV (50 μM), NADPH (1.0 mM), and each cDNA-expressed P450 enzyme (10 nM). Metabolites were analyzed by UPLC-TOFMS. The highest peak area of each metabolite produced by a cDNA-expressed P450 was set as 100%, and the contribution of others was compared with this enzyme. The data are expressed as means. The metabolite identification was accordant with Figs. 2 and 6.

IIIIIIVVVI
%
Control00000
CYP1A200000
CYP2A65.60000
CYP2B600000
CYP2C8013001.1
CYP2C900000
CYP2C19060000
CYP2D60100000
CYP2E100000
CYP3A410027100100100