TABLE 1

Summary of primers used for PCR amplification of CYP450 isoforms

Forward primers were modified by adding a Kozak sequence (GCC) immediately upstream of the start codon (underlined). Reverse primers were designed to eliminate the original stop codon of the protein sequence allowing for the incorporation of the V5-His-tag.

ProteinForward (5′-3′)Reverse (5′-3′)Plasmid NameReference Sequence
Human CYP2E1GCCATGTCTGCCCTCGGAGTCTGAGCGGGGAATGACACAGAGhCYP2E1NM_000773.3
Porcine CYP1A1GCCATGTTCTCTGTGTTTGGAAGAGCGCACATGCATCTGGACCYP1A1NM_214412
Porcine CYP2C33v4GCCATGGAGCTGCTGGGAATCTCTAGGGATGAGGCACAGCTTCYP2C33v4NM_214414
Porcine CYP2C49GCCATGGATGGGGCTGTGGTTACAGGAATGAAGCGGAGCTGCYP2C49NM_214420
Porcine CYP2A19GCCATGCTGGCCTCAGGCGCGGGGCTGGAAGCTCATGGTCYP2A19NM_214417
Porcine CYP3AGCCATGGACCTGATCCCAGGCTCCACTTACGGTCCCATCCYP3ANM_214423
Porcine CYP2E1GCCATGACTGCCCTGGGCCACTTGTGAGCGGGGAATGACCYP2E1AB052259